5OMD
Crystal structure of S. cerevisiae Ddc2 N-terminal coiled-coil domain
Summary for 5OMD
| Entry DOI | 10.2210/pdb5omd/pdb |
| Descriptor | DNA damage checkpoint protein LCD1 (2 entities in total) |
| Functional Keywords | coiled-coil, dimerization, protein binding |
| Biological source | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Cellular location | Cytoplasm: Q04377 |
| Total number of polymer chains | 1 |
| Total formula weight | 7733.98 |
| Authors | Deshpande, I.,Seeber, A.,Shimada, K.,Keusch, J.J.,Gut, H.,Gasser, S.M. (deposition date: 2017-07-28, release date: 2017-10-25, Last modification date: 2024-01-17) |
| Primary citation | Deshpande, I.,Seeber, A.,Shimada, K.,Keusch, J.J.,Gut, H.,Gasser, S.M. Structural Basis of Mec1-Ddc2-RPA Assembly and Activation on Single-Stranded DNA at Sites of Damage. Mol. Cell, 68:431-445.e5, 2017 Cited by PubMed Abstract: Mec1-Ddc2 (ATR-ATRIP) is a key DNA-damage-sensing kinase that is recruited through the single-stranded (ss) DNA-binding replication protein A (RPA) to initiate the DNA damage checkpoint response. Activation of ATR-ATRIP in the absence of DNA damage is lethal. Therefore, it is important that damage-specific recruitment precedes kinase activation, which is achieved at least in part by Mec1-Ddc2 homodimerization. Here, we report a structural, biochemical, and functional characterization of the yeast Mec1-Ddc2-RPA assembly. High-resolution co-crystal structures of Ddc2-Rfa1 and Ddc2-Rfa1-t11 (K45E mutant) N termini and of the Ddc2 coiled-coil domain (CCD) provide insight into Mec1-Ddc2 homodimerization and damage-site targeting. Based on our structural and functional findings, we present a Mec1-Ddc2-RPA-ssDNA composite structural model. By way of validation, we show that RPA-dependent recruitment of Mec1-Ddc2 is crucial for maintaining its homodimeric state at ssDNA and that Ddc2's recruitment domain and CCD are important for Mec1-dependent survival of UV-light-induced DNA damage. PubMed: 29033322DOI: 10.1016/j.molcel.2017.09.019 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.1 Å) |
Structure validation
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