5OMD
Crystal structure of S. cerevisiae Ddc2 N-terminal coiled-coil domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-06 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.00005 |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 116.940, 19.890, 30.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.980 - 2.100 |
R-factor | 0.2333 |
Rwork | 0.228 |
R-free | 0.26950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a92 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.150 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 4644 | 330 |
<I/σ(I)> | 13.23 | 2.19 |
Completeness [%] | 99.8 | 98.5 |
Redundancy | 6.08 | 5.72 |
CC(1/2) | 0.999 | 0.706 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris-HCl, 24% PEG 400 |