5OMD
Crystal structure of S. cerevisiae Ddc2 N-terminal coiled-coil domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-06 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.00005 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 116.940, 19.890, 30.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.980 - 2.100 |
| R-factor | 0.2333 |
| Rwork | 0.228 |
| R-free | 0.26950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a92 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 4644 | 330 |
| <I/σ(I)> | 13.23 | 2.19 |
| Completeness [%] | 99.8 | 98.5 |
| Redundancy | 6.08 | 5.72 |
| CC(1/2) | 0.999 | 0.706 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris-HCl, 24% PEG 400 |
