5OLN

X-Ray Structure of the Complex Pyrimidine-nucleoside phosphorylase from Bacillus subtilis at 1.88 A

Summary for 5OLN

Entry DOI	10.2210/pdb5oln/pdb
Related	5EP8
Descriptor	Pyrimidine-nucleoside phosphorylase, IMIDAZOLE, 1,2-ETHANEDIOL, ... (5 entities in total)
Functional Keywords	transferase, pyrimidine-nucleoside phosphorylase, np-2 superfamily
Biological source	Bacillus subtilis (strain 168)
Total number of polymer chains	2
Total formula weight	93031.83
Authors	Balaev, V.V.,Prokofev, I.I.,Gabdoulkhakov, A.G.,Betzel, C.,Lashkov, A.A. (deposition date: 2017-07-28, release date: 2018-04-18, Last modification date: 2024-01-17)
Primary citation	Balaev, V.V.,Prokofev, I.I.,Gabdoulkhakov, A.G.,Betzel, C.,Lashkov, A.A. Crystal structure of pyrimidine-nucleoside phosphorylase from Bacillus subtilis in complex with imidazole and sulfate. Acta Crystallogr F Struct Biol Commun, 74:193-197, 2018 Cited by PubMed Abstract: Pyrimidine-nucleoside phosphorylase catalyzes the phosphorolytic cleavage of thymidine and uridine with equal activity. Investigation of this protein is essential for anticancer drug design. Here, the structure of this protein from Bacillus subtilis in complex with imidazole and sulfate is reported at 1.9 Å resolution, which is an improvement on the previously reported structure at 2.6 Å resolution. The localization and position of imidazole in the nucleoside-binding site reflects the possible binding of ligands that possess an imidazole ring. PubMed: 29633966 DOI: 10.1107/S2053230X18002935 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.88 Å)

Structure validation