5OLN
X-Ray Structure of the Complex Pyrimidine-nucleoside phosphorylase from Bacillus subtilis at 1.88 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-13 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.368, 93.010, 139.854 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.505 - 1.880 |
R-factor | 0.2014 |
Rwork | 0.200 |
R-free | 0.23920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ep8 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER (2.6.1) |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 77.447 | 46.618 | 1.980 |
High resolution limit [Å] | 1.880 | 5.950 | 1.880 |
Rmerge | 0.067 | 1.055 | |
Rmeas | 0.207 | 0.070 | 1.103 |
Rpim | 0.062 | 0.022 | 0.320 |
Total number of observations | 755376 | 23166 | 112933 |
Number of reflections | 67839 | 2379 | 9725 |
<I/σ(I)> | 8.5 | 21.9 | 2.4 |
Completeness [%] | 98.4 | 99.5 | 97.7 |
Redundancy | 11.1 | 9.7 | 11.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.2 M Amonium acetate, 0.1 M Bis-tris |