5OC4

Crystal structure of human tRNA-dihydrouridine(20) synthase dsRBD R361A-R362A mutant

Summary for 5OC4

• Entry DOI: 10.2210/pdb5oc4/pdb
• Descriptor: tRNA-dihydrouridine(20) synthase [NAD(P)+]-like, CHLORIDE ION, GLYCEROL, ... (5 entities in total)
• Functional Keywords: double-stranded rna-binding domain, rna binding protein
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 13781.90

Authors

Bou-nader, C.,Pecqueur, L.,Hamdane, D. (deposition date: 2017-06-29, release date: 2018-12-26, Last modification date: 2024-01-17)

Primary citation

Bou-Nader, C.,Barraud, P.,Pecqueur, L.,Perez, J.,Velours, C.,Shepard, W.,Fontecave, M.,Tisne, C.,Hamdane, D.
Molecular basis for transfer RNA recognition by the double-stranded RNA-binding domain of human dihydrouridine synthase 2.
Nucleic Acids Res., 47:3117-3126, 2019

PubMed Abstract: Double stranded RNA-binding domain (dsRBD) is a ubiquitous domain specialized in the recognition of double-stranded RNAs (dsRNAs). Present in many proteins and enzymes involved in various functional roles of RNA metabolism, including RNA splicing, editing, and transport, dsRBD generally binds to RNAs that lack complex structures. However, this belief has recently been challenged by the discovery of a dsRBD serving as a major tRNA binding module for human dihydrouridine synthase 2 (hDus2), a flavoenzyme that catalyzes synthesis of dihydrouridine within the complex elbow structure of tRNA. We here unveil the molecular mechanism by which hDus2 dsRBD recognizes a tRNA ligand. By solving the crystal structure of this dsRBD in complex with a dsRNA together with extensive characterizations of its interaction with tRNA using mutagenesis, NMR and SAXS, we establish that while hDus2 dsRBD retains a conventional dsRNA recognition capability, the presence of an N-terminal extension appended to the canonical domain provides additional residues for binding tRNA in a structure-specific mode of action. Our results support that this extension represents a feature by which the dsRBD specializes in tRNA biology and more broadly highlight the importance of structural appendages to canonical domains in promoting the emergence of functional diversity.

PubMed: 30605527
DOI: 10.1093/nar/gky1302

Experimental method

X-RAY DIFFRACTION (1.71 Å)