5O7T

Crystal structure of KlenTaq mutant M747K in a closed ternary complex with a dG:dCTP base pair

Summary for 5O7T

Entry DOI	10.2210/pdb5o7t/pdb
Descriptor	DNA polymerase I, thermostable, DNA primer, DNA template, ... (7 entities in total)
Functional Keywords	dna polymerase, transferase
Biological source	Thermus aquaticus More
Total number of polymer chains	3
Total formula weight	70148.70
Authors	Betz, K.,Diederichs, K.,Marx, A. (deposition date: 2017-06-09, release date: 2017-11-29, Last modification date: 2024-01-17)
Primary citation	Betz, K.,Nilforoushan, A.,Wyss, L.A.,Diederichs, K.,Sturla, S.J.,Marx, A. Structural basis for the selective incorporation of an artificial nucleotide opposite a DNA adduct by a DNA polymerase. Chem. Commun. (Camb.), 53:12704-12707, 2017 Cited by PubMed Abstract: The possibility to sequence cytotoxic O-alkylG DNA adducts would greatly benefit research. Recently we reported a benzimidazole-derived nucleotide that is selectively incorporated opposite the damaged site by a mutated DNA polymerase. Here we provide the structural basis for this reaction which may spur future developments in DNA damage sequencing. PubMed: 29136072 DOI: 10.1039/c7cc07173f PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.8 Å)

Structure validation