5O7T
Crystal structure of KlenTaq mutant M747K in a closed ternary complex with a dG:dCTP base pair
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase I, thermostable | polymer | 540 | 60934.9 | 1 | UniProt (P19821) Pfam (PF09281) Pfam (PF00476) | Thermus aquaticus | Taq polymerase 1 |
| 2 | B (B) | DNA primer | polymer | 12 | 3657.4 | 1 | synthetic construct | ||
| 3 | C (C) | DNA template | polymer | 16 | 4924.2 | 1 | synthetic construct | ||
| 4 | D, E, G (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 5 | F (A) | 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE | non-polymer | 467.2 | 1 | Chemie (DCP) | |||
| 6 | H (B) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 7 | I, J, K (A, B, C) | water | water | 18.0 | 265 | Chemie (HOH) |
Sequence modifications
A: 293 - 832 (UniProt: P19821)
| PDB | External Database | Details |
|---|---|---|
| Lys 747 | Met 747 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 69516.5 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 632.2 | |
| All* | Total formula weight | 70148.7 |
