5O5P

Poliovirus type 3 (strain Saukett) stabilized virus-like particle in complex with the pocket factor compound GPP3

Summary for 5O5P

• Entry DOI: 10.2210/pdb5o5p/pdb
• EMDB information: 3749
• Descriptor: Capsid proteins, VP1, Capsid proteins, VP2, Capsid proteins, VP3, ... (5 entities in total)
• Functional Keywords: poliovirus, virus-like particle, vaccine, pocket factor, virus like particle
• Biological source: Human poliovirus 3; More
• Total number of polymer chains: 4
• Total formula weight: 97927.47

Authors

Bahar, M.W.,Kotecha, A.,Fry, E.E.,Stuart, D.I. (deposition date: 2017-06-02, release date: 2017-07-12, Last modification date: 2024-07-10)

Primary citation

Marsian, J.,Fox, H.,Bahar, M.W.,Kotecha, A.,Fry, E.E.,Stuart, D.I.,Macadam, A.J.,Rowlands, D.J.,Lomonossoff, G.P.
Plant-made polio type 3 stabilized VLPs-a candidate synthetic polio vaccine.
Nat Commun, 8:245-245, 2017

PubMed Abstract: Poliovirus (PV) is the causative agent of poliomyelitis, a crippling human disease known since antiquity. PV occurs in two distinct antigenic forms, D and C, of which only the D form elicits a robust neutralizing response. Developing a synthetically produced stabilized virus-like particle (sVLP)-based vaccine with D antigenicity, without the drawbacks of current vaccines, will be a major step towards the final eradication of poliovirus. Such a sVLP would retain the native antigenic conformation and the repetitive structure of the original virus particle, but lack infectious genomic material. In this study, we report the production of synthetically stabilized PV VLPs in plants. Mice carrying the gene for the human PV receptor are protected from wild-type PV when immunized with the plant-made PV sVLPs. Structural analysis of the stabilized mutant at 3.6 Å resolution by cryo-electron microscopy and single-particle reconstruction reveals a structure almost indistinguishable from wild-type PV3.Despite the success of current vaccination against poliomyelitis, safe, cheap and effective vaccines remain sought for continuing eradication effort. Here the authors use plants to express stabilized virus-like particles of type 3 poliovirus that can induce a protective immune response in mice transgenic for the human poliovirus receptor.

PubMed: 28811473
DOI: 10.1038/s41467-017-00090-w

Experimental method

ELECTRON MICROSCOPY (4.1 Å)