5O1S

Dimethyl fumarate is an allosteric covalent inhibitor of the p90 ribosomal S6 kinases

Summary for 5O1S

• Entry DOI: 10.2210/pdb5o1s/pdb
• Descriptor: Ribosomal protein S6 kinase alpha-3, SODIUM ION, Dimethyl fumarate, ... (4 entities in total)
• Functional Keywords: covalent, inhibitor, kinase, allosteric, transferase
• Biological source: Mus musculus (Mouse)
• Total number of polymer chains: 1
• Total formula weight: 41557.10

Authors

Andersen, J.L.,Nissen, P. (deposition date: 2017-05-19, release date: 2018-05-30, Last modification date: 2024-10-09)

Primary citation

Andersen, J.L.,Gesser, B.,Funder, E.D.,Nielsen, C.J.F.,Gotfred-Rasmussen, H.,Rasmussen, M.K.,Toth, R.,Gothelf, K.V.,Arthur, J.S.C.,Iversen, L.,Nissen, P.
Dimethyl fumarate is an allosteric covalent inhibitor of the p90 ribosomal S6 kinases.
Nat Commun, 9:4344-4344, 2018

PubMed Abstract: Dimethyl fumarate (DMF) has been applied for decades in the treatment of psoriasis and now also multiple sclerosis. However, the mechanism of action has remained obscure and involves high dose over long time of this small, reactive compound implicating many potential targets. Based on a 1.9 Å resolution crystal structure of the C-terminal kinase domain of the mouse p90 Ribosomal S6 Kinase 2 (RSK2) inhibited by DMF we describe a central binding site in RSKs and the closely related Mitogen and Stress-activated Kinases (MSKs). DMF reacts covalently as a Michael acceptor to a conserved cysteine residue in the αF-helix of RSK/MSKs. Binding of DMF prevents the activation loop of the kinase from engaging substrate, and stabilizes an auto-inhibitory αL-helix, thus pointing to an effective, allosteric mechanism of kinase inhibition. The biochemical and cell biological characteristics of DMF inhibition of RSK/MSKs are consistent with the clinical protocols of DMF treatment.

PubMed: 30341347
DOI: 10.1038/s41467-018-06787-w

Experimental method

X-RAY DIFFRACTION (1.9 Å)