5NSL

Structure of D80A-fructofuranosidase from Xanthophyllomyces dendrorhous complexed with fructose and hydroxytyrosol

Summary for 5NSL

• Entry DOI: 10.2210/pdb5nsl/pdb
• Related: 5ANN 5FIX 5FK7 5FK8 5FKB 5FKC 5FMB 5FMC 5FMD
• Descriptor: Beta-fructofuranosidase, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (9 entities in total)
• Functional Keywords: hydrolase, carbohydrates, catalysis, catalytic domain, cloning, dimerization, glycoside hydrolases, fungal proteins, kinetics, substrate specificity, beta-fructofuranosidase, invertase, transglycosylation, polyphenol, antioxidant, hydroxityrosol
• Biological source: Phaffia rhodozyma (Yeast)
• Total number of polymer chains: 2
• Total formula weight: 165286.09

Authors

Ramirez-Escudero, M.,Sanz-Aparicio, J. (deposition date: 2017-04-26, release date: 2018-11-07, Last modification date: 2024-10-16)

• Primary citation: Miquez, N.,Ramirez-Escudero, M.,Poveda, A.,Ballesteros, O.,Sanz-Aparicio, J.,Plou, F.J.; Fructosylation of Hydroxytyrosol by the Beta-Fructofuranosidase from Xanthophyllomyces dendrorhous: Insights into the Molecular Basis of the Enzyme Specificity; Chemcatchem, 2018; DOI: 10.1002/cctc.201801171

Experimental method

X-RAY DIFFRACTION (1.7 Å)