5NQU

Tubulin Darpin cryo structure

Summary for 5NQU

DescriptorTubulin alpha-1B chain, Tubulin beta-2B chain, Designed Ankyrin Repeat Protein (DARPIN) D1, ... (7 entities in total)
Functional Keywordsroom-temperature, serial crystallography, cell cycle
Biological sourcesynthetic construct
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Total number of polymer chains3
Total molecular weight119263.46
Authors
Weinert, T.,Olieric, N.,James, D.,Gashi, D.,Nogly, P.,Jaeger, K.,Steinmetz, M.O.,Standfuss, J. (deposition date: 2017-04-21, release date: 2017-09-27, Last modification date: 2019-08-14)
Primary citation
Weinert, T.,Olieric, N.,Cheng, R.,Brunle, S.,James, D.,Ozerov, D.,Gashi, D.,Vera, L.,Marsh, M.,Jaeger, K.,Dworkowski, F.,Panepucci, E.,Basu, S.,Skopintsev, P.,Dore, A.S.,Geng, T.,Cooke, R.M.,Liang, M.,Prota, A.E.,Panneels, V.,Nogly, P.,Ermler, U.,Schertler, G.,Hennig, M.,Steinmetz, M.O.,Wang, M.,Standfuss, J.
Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons.
Nat Commun, 8:542-542, 2017
PubMed: 28912485 (PDB entries with the same primary citation)
DOI: 10.1038/s41467-017-00630-4
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.8 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.20810 2.6% 7.1%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution