5NQU
Tubulin Darpin cryo structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-26 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.040, 91.740, 82.645 |
| Unit cell angles | 90.00, 97.46, 90.00 |
Refinement procedure
| Resolution | 26.350 - 1.800 |
| R-factor | 0.177 |
| Rwork | 0.176 |
| R-free | 0.20700 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.200 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmeas | 0.108 | 2.202 |
| Number of reflections | 100723 | 7249 |
| <I/σ(I)> | 11.6 | 0.7 |
| Completeness [%] | 99.7 | 97.2 |
| Redundancy | 6.8 | 6.21 |
| CC(1/2) | 0.427 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 20-24% PEG 3350, 0.2 M Ammonium Sulfate, 0.1 M Bis-Tris Methane pH5.5 |
