Summary for 5NP6
| Entry DOI | 10.2210/pdb5np6/pdb |
| EMDB information | 3618 |
| Descriptor | mRNA, 30S ribosomal protein S7, 30S ribosomal protein S8, ... (57 entities in total) |
| Functional Keywords | ribosome, translation, bypassing, protein |
| Biological source | Escherichia coli (strain K12) More |
| Total number of polymer chains | 57 |
| Total formula weight | 2180309.45 |
| Authors | Agirrezabala, X.,Samatova, E.,Klimova, M.,Zamora, M.,Gil-Carton, D.,Rodnina, M.,Valle, M. (deposition date: 2017-04-13, release date: 2017-06-14, Last modification date: 2025-03-19) |
| Primary citation | Agirrezabala, X.,Samatova, E.,Klimova, M.,Zamora, M.,Gil-Carton, D.,Rodnina, M.V.,Valle, M. Ribosome rearrangements at the onset of translational bypassing. Sci Adv, 3:e1700147-e1700147, 2017 Cited by PubMed Abstract: Bypassing is a recoding event that leads to the translation of two distal open reading frames into a single polypeptide chain. We present the structure of a translating ribosome stalled at the bypassing take-off site of of bacteriophage T4. The nascent peptide in the exit tunnel anchors the P-site peptidyl-tRNA to the ribosome and locks an inactive conformation of the peptidyl transferase center (PTC). The mRNA forms a short dynamic hairpin in the decoding site. The ribosomal subunits adopt a rolling conformation in which the rotation of the small subunit around its long axis causes the opening of the A-site region. Together, PTC conformation and mRNA structure safeguard against premature termination and read-through of the stop codon and reconfigure the ribosome to a state poised for take-off and sliding along the noncoding mRNA gap. PubMed: 28630923DOI: 10.1126/sciadv.1700147 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.6 Å) |
Structure validation
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