5NNC

Crystal Structure of the first bromodomain of human BRD4 in complex with a diacetylated histone 4 peptide (H3K9ac/K14ac)

Summary for 5NNC

DescriptorBromodomain-containing protein 4, Histone H3 (3 entities in total)
Functional Keywordsbromodomain, transcription, complex
Biological sourceHomo sapiens (Human)
Total number of polymer chains4
Total molecular weight34185.3
Authors
Filippakopoulos, P.,Picaud, S.,Pike, A.C.W.,von Delft, F.,Arrowsmith, C.H.,Edwards, A.M.,Bountra, C. (deposition date: 2017-04-08, release date: 2018-05-16, Last modification date: 2019-02-20)
Primary citation
Lambert, J.P.,Picaud, S.,Fujisawa, T.,Hou, H.,Savitsky, P.,Uuskula-Reimand, L.,Gupta, G.D.,Abdouni, H.,Lin, Z.Y.,Tucholska, M.,Knight, J.D.R.,Gonzalez-Badillo, B.,St-Denis, N.,Newman, J.A.,Stucki, M.,Pelletier, L.,Bandeira, N.,Wilson, M.D.,Filippakopoulos, P.,Gingras, A.C.
Interactome Rewiring Following Pharmacological Targeting of BET Bromodomains.
Mol. Cell, 73:621-638.e17, 2019
PubMed: 30554943 (PDB entries with the same primary citation)
DOI: 10.1016/j.molcel.2018.11.006
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.22 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.2695 0.4% 2.7% 1.5%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 5nnc
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Molmil generated image of 5nnc
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Molmil generated image of 5nnc
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More Biological unit images

Molmil generated image of 5nnc
no rotation
Molmil generated image of 5nnc
rotated about x axis by 90°
Molmil generated image of 5nnc
rotated about y axis by 90°
(*)In the case of coarse surface representation, the asymmetric unit is shown as red ribbon representation.
Coordinate files for Biological unit (5nnc.pdb1.gz [45.92 KB])
Coordinate files for Biological unit (5nnc.pdb2.gz [45.97 KB])