5N7L
Crystal structure of the periplasmic domain of XcpY, tI crystal form.
Summary for 5N7L
| Entry DOI | 10.2210/pdb5n7l/pdb |
| Descriptor | Type II secretion system protein L, BROMIDE ION, GLUTAMIC ACID, ... (4 entities in total) |
| Functional Keywords | periplasm, ferrodoxin fold, gspl protein, protein transport |
| Biological source | Pseudomonas aeruginosa PAO1 |
| Total number of polymer chains | 1 |
| Total formula weight | 8793.22 |
| Authors | Fulara, A.,Savvides, S.N. (deposition date: 2017-02-20, release date: 2018-03-14, Last modification date: 2024-01-17) |
| Primary citation | Fulara, A.,Vandenberghe, I.,Read, R.J.,Devreese, B.,Savvides, S.N. Structure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa. Sci Rep, 8:16760-16760, 2018 Cited by PubMed Abstract: The ability of bacteria to infect a host relies in part on the secretion of molecular virulence factors across the cell envelope. Pseudomonas aeruginosa, a ubiquitous environmental bacterium causing opportunistic infections in humans, employs the type II secretion system (T2SS) to transport effector proteins across its cellular envelope as part of a diverse array of virulence strategies. General secretory pathway protein L (GspL) is an essential inner-membrane component of the T2SS apparatus, and is thought to facilitate transduction of the energy from ATP hydrolysis in the cytoplasm to the periplasmic components of the system. However, our incomplete understanding of the assembly principles of the T2SS machinery prevents the mechanistic deconvolution of T2SS-mediated protein secretion. Here we show via two crystal structures that the periplasmic ferredoxin-like domain of GspL (GspL) is a dimer stabilized by hydrophobic interactions, and that this interface may allow significant interdomain plasticity. The general dimerization mode of GspL is shared with GspL from Vibrio parahaemolyticus suggesting a conserved oligomerization mode across the GspL family. Furthermore, we identified a tetrameric form of the complete periplasmic segment of GspL (GspL) which indicates that GspL may be able to adopt multiple oligomeric states as part of its dynamic role in the T2SS apparatus. PubMed: 30425318DOI: 10.1038/s41598-018-34956-w PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.501 Å) |
Structure validation
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