5N7L
Crystal structure of the periplasmic domain of XcpY, tI crystal form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-31 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 100.439, 100.439, 98.848 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.894 - 2.501 |
| R-factor | 0.2031 |
| Rwork | 0.201 |
| R-free | 0.22040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5N7S |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.805 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.000 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmeas | 0.130 | |
| Number of reflections | 9039 | 1419 |
| <I/σ(I)> | 16.56 | |
| Completeness [%] | 99.7 | 98.5 |
| Redundancy | 12.97 | 12.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.3 M KBr or 0.2 M MgCl2, 0.1 M sodium cacodylate, pH=6.5, gamma-PGA |
