5MYY

Hen Egg-White Lysozyme (HEWL) cocrystallized in the presence of Cadmium sulphate

Summary for 5MYY

• Entry DOI: 10.2210/pdb5myy/pdb
• Descriptor: Lysozyme C, CADMIUM ION, CHLORIDE ION, ... (6 entities in total)
• Functional Keywords: sad phasing, cadmium phasing, lysozyme, hydrolase
• Biological source: Gallus gallus (Chicken)
• Total number of polymer chains: 1
• Total formula weight: 15894.86

Authors

Panneerselvam, S.,Burkhardt, A.,Meents, A. (deposition date: 2017-01-30, release date: 2017-07-12, Last modification date: 2024-11-06)

Primary citation

Panneerselvam, S.,Kumpula, E.P.,Kursula, I.,Burkhardt, A.,Meents, A.
Rapid cadmium SAD phasing at the standard wavelength (1 angstrom ).
Acta Crystallogr D Struct Biol, 73:581-590, 2017

PubMed Abstract: Cadmium ions can be effectively used to promote crystal growth and for experimental phasing. Here, the use of cadmium ions as a suitable anomalous scatterer at the standard wavelength of 1 Å is demonstrated. The structures of three different proteins were determined using cadmium single-wavelength anomalous dispersion (SAD) phasing. Owing to the strong anomalous signal, the structure of lysozyme could be automatically phased and built using a very low anomalous multiplicity (1.1) and low-completeness (77%) data set. Additionally, it is shown that cadmium ions can easily substitute divalent ions in ATP-divalent cation complexes. This property could be generally applied for phasing experiments of a wide range of nucleotide-binding proteins. Improvements in crystal growth and quality, good anomalous signal at standard wavelengths (i.e. no need to change photon energy) and rapid phasing and refinement using a single data set are benefits that should allow cadmium ions to be widely used for experimental phasing.

PubMed: 28695858
DOI: 10.1107/S2059798317006970

Experimental method

X-RAY DIFFRACTION (1.1 Å)