5MYY
Hen Egg-White Lysozyme (HEWL) cocrystallized in the presence of Cadmium sulphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2015-10-22 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.03320 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.740, 78.740, 37.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.680 - 1.100 |
R-factor | 0.13013 |
Rwork | 0.130 |
R-free | 0.14305 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.620 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXDE |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.370 |
High resolution limit [Å] | 1.100 |
Number of reflections | 47744 |
<I/σ(I)> | 55 |
Completeness [%] | 100.0 |
Redundancy | 28 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1 M Sodium acetate pH 4.8 10-12% NaCl 20-25% Ethylene Glycol 60 mg/ ml HEWL in 0.1 M Sodium acetate pH 4.8 |