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5MUJ

BT0996 RGII Chain B Complex

Summary for 5MUJ
Entry DOI10.2210/pdb5muj/pdb
DescriptorBeta-galactosidase, alpha-L-rhamnopyranose-(1-2)-[alpha-L-rhamnopyranose-(1-3)]alpha-L-arabinopyranose-(1-4)-[4-O-[(1R)-1-hydroxyethyl]-2-O-methyl-alpha-L-fucopyranose-(1-2)]beta-D-galactopyranose-(1-2)-alpha-D-aceric acid-(1-4)-alpha-L-rhamnopyranose-(1-3)-3-C-(hydroxylmethyl)-alpha-D-erythrofuranose, ACETATE ION, ... (4 entities in total)
Functional Keywordsglycoside hydrolase, arabinofuranosidase, plant pectin, rgii, hydrolase
Biological sourceBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Total number of polymer chains1
Total formula weight45454.40
Authors
Cartmell, A.,Basle, A.,Ndeh, D.,Luis, A.S.,Venditto, I.,Labourel, A.,Rogowski, A.,Gilbert, H.J. (deposition date: 2017-01-13, release date: 2017-04-05, Last modification date: 2024-11-06)
Primary citationNdeh, D.,Rogowski, A.,Cartmell, A.,Luis, A.S.,Basle, A.,Gray, J.,Venditto, I.,Briggs, J.,Zhang, X.,Labourel, A.,Terrapon, N.,Buffetto, F.,Nepogodiev, S.,Xiao, Y.,Field, R.A.,Zhu, Y.,O'Neill, M.A.,Urbanowicz, B.R.,York, W.S.,Davies, G.J.,Abbott, D.W.,Ralet, M.C.,Martens, E.C.,Henrissat, B.,Gilbert, H.J.
Complex pectin metabolism by gut bacteria reveals novel catalytic functions.
Nature, 544:65-70, 2017
Cited by
PubMed Abstract: The metabolism of carbohydrate polymers drives microbial diversity in the human gut microbiota. It is unclear, however, whether bacterial consortia or single organisms are required to depolymerize highly complex glycans. Here we show that the gut bacterium Bacteroides thetaiotaomicron uses the most structurally complex glycan known: the plant pectic polysaccharide rhamnogalacturonan-II, cleaving all but 1 of its 21 distinct glycosidic linkages. The deconstruction of rhamnogalacturonan-II side chains and backbone are coordinated to overcome steric constraints, and the degradation involves previously undiscovered enzyme families and catalytic activities. The degradation system informs revision of the current structural model of rhamnogalacturonan-II and highlights how individual gut bacteria orchestrate manifold enzymes to metabolize the most challenging glycan in the human diet.
PubMed: 28329766
DOI: 10.1038/nature21725
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.37 Å)
Structure validation

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