5MRF

Structure of the yeast mitochondrial ribosome - Class C

これはPDB形式変換不可エントリーです。

5MRF の概要

• エントリーDOI: 10.2210/pdb5mrf/pdb
• EMDBエントリー: 2566 3553
• 分子名称: 21S ribosomal RNA, uL15m, uL16m, ... (82 entities in total)
• 機能のキーワード: yeast, mitochondrial, ribosome, saccharomyces cerevisiae
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 78
• 化学式量合計: 3325585.75

構造登録者

Desai, N.,Brown, A.,Amunts, A.,Ramakrishnan, V. (登録日: 2016-12-22, 公開日: 2017-02-15, 最終更新日: 2024-05-15)

主引用文献

Desai, N.,Brown, A.,Amunts, A.,Ramakrishnan, V.
The structure of the yeast mitochondrial ribosome.
Science, 355:528-531, 2017

PubMed Abstract: Mitochondria have specialized ribosomes (mitoribosomes) dedicated to the expression of the genetic information encoded by their genomes. Here, using electron cryomicroscopy, we have determined the structure of the 75-component yeast mitoribosome to an overall resolution of 3.3 angstroms. The mitoribosomal small subunit has been built de novo and includes 15S ribosomal RNA (rRNA) and 34 proteins, including 14 without homologs in the evolutionarily related bacterial ribosome. Yeast-specific rRNA and protein elements, including the acquisition of a putatively active enzyme, give the mitoribosome a distinct architecture compared to the mammalian mitoribosome. At an expanded messenger RNA channel exit, there is a binding platform for translational activators that regulate translation in yeast but not mammalian mitochondria. The structure provides insights into the evolution and species-specific specialization of mitochondrial translation.

PubMed: 28154081
DOI: 10.1126/science.aal2415

実験手法

ELECTRON MICROSCOPY (4.97 Å)