Summary for 5MJV
| Entry DOI | 10.2210/pdb5mjv/pdb |
| Descriptor | CAPSID SUBUNIT VP1, CAPSID SUBUNIT VP3, CAPSID SUBUNIT VP0, ... (4 entities in total) |
| Functional Keywords | virus, parechovirus, picornavirus, rna, human parechovirus 1, hpev1, hpev |
| Biological source | Human parechovirus 1 (strain Harris) (HPeV-1) More |
| Cellular location | Protein VP3: Virion . Protein VP1: Virion . Protein 2B: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Protein 2C: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Protein 3A: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Protein 3B: Virion . Protease 3C: Host cytoplasm . RNA-directed RNA polymerase 3D-POL: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side : Q66578 Q66578 Q66578 |
| Total number of polymer chains | 4 |
| Total formula weight | 88235.17 |
| Authors | Shakeel, S.,Dykeman, E.C.,White, S.J.,Ora, A.,Cockburn, J.J.B.,Butcher, S.J.,Stockley, P.G.,Twarock, R. (deposition date: 2016-12-01, release date: 2017-01-11, Last modification date: 2024-01-17) |
| Primary citation | Shakeel, S.,Dykeman, E.C.,White, S.J.,Ora, A.,Cockburn, J.J.,Butcher, S.J.,Stockley, P.G.,Twarock, R. Genomic RNA folding mediates assembly of human parechovirus. Nat Commun, 8:5-5, 2017 Cited by PubMed Abstract: Assembly of the major viral pathogens of the Picornaviridae family is poorly understood. Human parechovirus 1 is an example of such viruses that contains 60 short regions of ordered RNA density making identical contacts with the protein shell. We show here via a combination of RNA-based systematic evolution of ligands by exponential enrichment, bioinformatics analysis and reverse genetics that these RNA segments are bound to the coat proteins in a sequence-specific manner. Disruption of either the RNA coat protein recognition motif or its contact amino acid residues is deleterious for viral assembly. The data are consistent with RNA packaging signals playing essential roles in virion assembly. Their binding sites on the coat proteins are evolutionarily conserved across the Parechovirus genus, suggesting that they represent potential broad-spectrum anti-viral targets.The mechanism underlying packaging of genomic RNA into viral particles is not well understood for human parechoviruses. Here the authors identify short RNA motifs in the parechovirus genome that bind capsid proteins, providing approximately 60 specific interactions for virion assembly. PubMed: 28232749DOI: 10.1038/s41467-016-0011-z PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.09 Å) |
Structure validation
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