5ME3
Structure of the Scc2 C-terminus
Summary for 5ME3
| Entry DOI | 10.2210/pdb5me3/pdb |
| Descriptor | Sister chromatid cohesion protein 2, unassigned sequence of Scc2, Scc2 unassigned sequence, ... (4 entities in total) |
| Functional Keywords | cohesin loader, heat repeat, scc2, cell cycle |
| Biological source | Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) More |
| Cellular location | Nucleus : Q750S2 |
| Total number of polymer chains | 6 |
| Total formula weight | 268810.67 |
| Authors | Chao, W.C.H.,Singleton, M.R. (deposition date: 2016-11-14, release date: 2017-01-11, Last modification date: 2024-05-08) |
| Primary citation | Chao, W.C.,Murayama, Y.,Munoz, S.,Jones, A.W.,Wade, B.O.,Purkiss, A.G.,Hu, X.W.,Borg, A.,Snijders, A.P.,Uhlmann, F.,Singleton, M.R. Structure of the cohesin loader Scc2. Nat Commun, 8:13952-13952, 2017 Cited by PubMed Abstract: The functions of cohesin are central to genome integrity, chromosome organization and transcription regulation through its prevention of premature sister-chromatid separation and the formation of DNA loops. The loading of cohesin onto chromatin depends on the Scc2-Scc4 complex; however, little is known about how it stimulates the cohesion-loading activity. Here we determine the large 'hook' structure of Scc2 responsible for catalysing cohesin loading. We identify key Scc2 surfaces that are crucial for cohesin loading in vivo. With the aid of previously determined structures and homology modelling, we derive a pseudo-atomic structure of the full-length Scc2-Scc4 complex. Finally, using recombinantly purified Scc2-Scc4 and cohesin, we performed crosslinking mass spectrometry and interaction assays that suggest Scc2-Scc4 uses its modular structure to make multiple contacts with cohesin. PubMed: 28059076DOI: 10.1038/ncomms13952 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.85 Å) |
Structure validation
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