5M29

Structure of cobinamide-bound BtuF, the periplasmic vitamin B12 binding protein in E.coli

Summary for 5M29

• Entry DOI: 10.2210/pdb5m29/pdb
• Related: 1N2Z 1N4A 1N4D
• Descriptor: Vitamin B12-binding protein, COB(II)INAMIDE, CYANIDE ION, ... (5 entities in total)
• Functional Keywords: btuf, cobinamide, periplasmic binding protein, abc transporter, transport protein
• Biological source: Escherichia coli K-12
• Cellular location: Periplasm : P37028
• Total number of polymer chains: 2
• Total formula weight: 66003.06

Authors

Mireku, S.A.,Ruetz, M.,Zhou, T.,Korkhov, V.M.,Kraeutler, B.,Locher, K.P. (deposition date: 2016-10-12, release date: 2017-02-08, Last modification date: 2024-02-07)

• Primary citation: Mireku, S.A.,Ruetz, M.,Zhou, T.,Korkhov, V.M.,Krautler, B.,Locher, K.P.; Conformational Change of a Tryptophan Residue in BtuF Facilitates Binding and Transport of Cobinamide by the Vitamin B12 Transporter BtuCD-F.; Sci Rep, 7:41575-41575, 2017; PubMed: 28128319; DOI: 10.1038/srep41575

Experimental method

X-RAY DIFFRACTION (1.5 Å)