5M1J

Nonstop ribosomal complex bound with Dom34 and Hbs1

This is a large structure.

Summary for 5M1J

EMDB information4140
DescriptorProtein DOM34, 40S ribosomal protein S0-A, 40S ribosomal protein S26-B, ... (87 entities in total)
Functional Keywordsmrna surveillance, ribosome
Biological sourceSaccharomyces cerevisiae (Baker's yeast)
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Cellular locationCytoplasm  P33309 P32905 P39939 P33442 P35997 P25443 P0C0X0 P05750 P41057 P0CX35 P0CX33 P26783 P0CX37 P38011 P26786 P0CX39 O13516 Q08745 P0CX47 P48589 P05756 P06367 Q01855 P0CX51 P14127 P0CX55 P07280 P38701 P0C0V8 P0C0W1 P0CX29 P0CX31 Q3E792 P02406 P0CX45 P05747 P14126 P14120 P10664 P26321 P0C2H8 P38061 P05744 P05737 P87262 P17076 P0CX84 P05738 P05745 P41805 P0C0W9 P49166 P49167 P04650 Q12690 P36105 P05748 P0CX28 P0CX26 P05740 P0CX49 P0CX23 P05749 P0CX41 P0C2H6 P26784 P0CX87 P0CX82 P04456 P05743 Q02753 Q02326 P04449 P32769
Ubiquitin: Cytoplasm . 40S ribosomal protein S31: Cytoplasm  P05759
Ubiquitin: Cytoplasm . 60S ribosomal protein L40-A: Cytoplasm  P0CH08
Total number of polymer chains82
Total molecular weight3195268.1
Authors
Hilal, T.,Yamamoto, H.,Loerke, J.,Buerger, J.,Mielke, T.,Spahn, C.M.T. (deposition date: 2016-10-07, release date: 2017-01-18, Last modification date: 2017-08-02)
Primary citation
Hilal, T.,Yamamoto, H.,Loerke, J.,Burger, J.,Mielke, T.,Spahn, C.M.
Structural insights into ribosomal rescue by Dom34 and Hbs1 at near-atomic resolution.
Nat Commun, 7:13521-13521, 2016
PubMed: 27995908 (PDB entries with the same primary citation)
DOI: 10.1038/ncomms13521
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY (3.3 Å)
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Structure validation

ClashscoreRamachandran outliersSidechain outliersRNA backbone40.1%0.2%0.58MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures

More Asymmetric unit images

Molmil generated image of 5m1j
no rotation
Molmil generated image of 5m1j
rotated about x axis by 90°
Molmil generated image of 5m1j
rotated about y axis by 90°
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