5LN3

The human 26S Proteasome at 6.8 Ang.

5LN3 の概要

• エントリーDOI: 10.2210/pdb5ln3/pdb
• EMDBエントリー: 4089
• 分子名称: 26S proteasome non-ATPase regulatory subunit 2, Proteasome subunit alpha type-2, Proteasome subunit alpha type-4, ... (32 entities in total)
• 機能のキーワード: 26s proteasome, cryo-em, single particle analysis, homology modelling, hydrolase
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Cytoplasm : P25787 P25789 O14818 P28066 P25786 P25788 P35998 P62191 P62195 P43686 P28072 P62333 P17980 Q99436 P49720 P49721 P28074 P20618 P28070 P60900; Nucleus : O00231
• タンパク質・核酸の鎖数: 32
• 化学式量合計: 1282257.20

構造登録者

Schweitzer, A.,Beck, F.,Sakata, E.,Unverdorben, P. (登録日: 2016-08-03, 公開日: 2017-03-22, 最終更新日: 2024-05-15)

主引用文献

Wang, X.,Cimermancic, P.,Yu, C.,Schweitzer, A.,Chopra, N.,Engel, J.L.,Greenberg, C.,Huszagh, A.S.,Beck, F.,Sakata, E.,Yang, Y.,Novitsky, E.J.,Leitner, A.,Nanni, P.,Kahraman, A.,Guo, X.,Dixon, J.E.,Rychnovsky, S.D.,Aebersold, R.,Baumeister, W.,Sali, A.,Huang, L.
Molecular Details Underlying Dynamic Structures and Regulation of the Human 26S Proteasome.
Mol. Cell Proteomics, 16:840-854, 2017

PubMed Abstract: The 26S proteasome is the macromolecular machine responsible for ATP/ubiquitin dependent degradation. As aberration in proteasomal degradation has been implicated in many human diseases, structural analysis of the human 26S proteasome complex is essential to advance our understanding of its action and regulation mechanisms. In recent years, cross-linking mass spectrometry (XL-MS) has emerged as a powerful tool for elucidating structural topologies of large protein assemblies, with its unique capability of studying protein complexes in cells. To facilitate the identification of cross-linked peptides, we have previously developed a robust amine reactive sulfoxide-containing MS-cleavable cross-linker, disuccinimidyl sulfoxide (DSSO). To better understand the structure and regulation of the human 26S proteasome, we have established new DSSO-based and XL-MS workflows by coupling with HB-tag based affinity purification to comprehensively examine protein-protein interactions within the 26S proteasome. In total, we have identified 447 unique lysine-to-lysine linkages delineating 67 interprotein and 26 intraprotein interactions, representing the largest cross-link dataset for proteasome complexes. In combination with EM maps and computational modeling, the architecture of the 26S proteasome was determined to infer its structural dynamics. In particular, three proteasome subunits Rpn1, Rpn6, and Rpt6 displayed multiple conformations that have not been previously reported. Additionally, cross-links between proteasome subunits and 15 proteasome interacting proteins including 9 known and 6 novel ones have been determined to demonstrate their physical interactions at the amino acid level. Our results have provided new insights on the dynamics of the 26S human proteasome and the methodologies presented here can be applied to study other protein complexes.

PubMed: 28292943
DOI: 10.1074/mcp.M116.065326

実験手法

ELECTRON MICROSCOPY (6.8 Å)