Crystal structure of DARPin-DARPin rigid fusion, variant DD_D12_13_D12

Summary for 5LE7

DescriptorDD_D12_13_D12, SULFATE ION (3 entities in total)
Functional Keywordsde novo protein, designed ankyrin repeat proteins, protein design, protein engineering, rigid domain fusions
Biological sourcesynthetic construct
Total number of polymer chains4
Total molecular weight141253.6
Batyuk, A.,Wu, Y.,Mittl, P.R.,Plueckthun, A. (deposition date: 2016-06-29, release date: 2017-08-02, Last modification date: 2019-03-20)
Primary citation
Wu, Y.,Batyuk, A.,Honegger, A.,Brandl, F.,Mittl, P.R.E.,Pluckthun, A.
Rigidly connected multispecific artificial binders with adjustable geometries.
Sci Rep, 7:11217-11217, 2017
PubMed: 28894181 (PDB entries with the same primary citation)
DOI: 10.1038/s41598-017-11472-x
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.25700 0.5% 5.0%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 5le7
no rotation
Molmil generated image of 5le7
rotated about x axis by 90°
Molmil generated image of 5le7
rotated about y axis by 90°