5LE7

Crystal structure of DARPin-DARPin rigid fusion, variant DD_D12_13_D12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-10-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	147.940, 75.250, 131.830
Unit cell angles	90.00, 96.94, 90.00

Refinement procedure

Resolution	46.161 - 2.104
R-factor	0.2271
Rwork	0.225
R-free	0.25790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1SVX chain A
RMSD bond length	0.003
RMSD bond angle	0.489
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2386))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.161	46.161
High resolution limit [Å]	2.104	2.104
Rmerge	0.108	2.694
Number of reflections	83045
<I/σ(I)>	7.36
Completeness [%]	99.2
Redundancy	3.7
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	PEG 3350 25% w/v, (NH4)2SO4 0.2 M, Bis-Tris 0.1 M, pH 5.5