5LDD

Crystal structure of the heterodimeric GEF Mon1-Ccz1 in complex with Ypt7

5LDD の概要

• エントリーDOI: 10.2210/pdb5ldd/pdb
• 分子名称: Mon1, Ccz1, Rab small monomeric GTPase-like protein, ... (5 entities in total)
• 機能のキーワード: heterodimeric gef protein, endosomal maturation, rab gtpase, protein transport
• 由来する生物種: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 138556.91

構造登録者

Kiontke, S.,Kuemmel, D. (登録日: 2016-06-24, 公開日: 2017-01-18, 最終更新日: 2018-03-14)

主引用文献

Kiontke, S.,Langemeyer, L.,Kuhlee, A.,Schuback, S.,Raunser, S.,Ungermann, C.,Kummel, D.
Architecture and mechanism of the late endosomal Rab7-like Ypt7 guanine nucleotide exchange factor complex Mon1-Ccz1.
Nat Commun, 8:14034-14034, 2017

PubMed Abstract: The Mon1-Ccz1 complex (MC1) is the guanine nucleotide exchange factor (GEF) for the Rab GTPase Ypt7/Rab7 and is required for endosomal maturation and fusion at the vacuole/lysosome. Here we present the overall architecture of MC1 from Chaetomium thermophilum, and in combining biochemical studies and mutational analysis in yeast, we identify the domains required for catalytic activity, complex assembly and localization of MC1. The crystal structure of a catalytic MC1 core complex bound to Ypt7 provides mechanistic insight into its function. We pinpoint the determinants that allow for a discrimination of the Rab7-like Ypt7 over the Rab5-like Vps21, which are both located on the same membrane. MC1 shares structural similarities with the TRAPP complex, but employs a novel mechanism to promote nucleotide exchange that utilizes a conserved lysine residue of Ypt7, which is inserted upon MC1 binding into the nucleotide-binding pocket of Ypt7 and contributes to specificity.

PubMed: 28051187
DOI: 10.1038/ncomms14034

実験手法

X-RAY DIFFRACTION (2.496 Å)