5LBC

HIF prolyl hydroxylase 2 (PHD2/EGLN1) I280V/R281L/I292V variant in complex with Mn(II) and N-[(1-chloro-4-hydroxyisoquinolin-3-yl)carbonyl]glycine (IOX3/FG2216)

5LBC の概要

• エントリーDOI: 10.2210/pdb5lbc/pdb
• 関連するPDBエントリー: 3HQR 4BQX 5L9B 5L9R 5L9V 5LA9 5LAS 5LAT 5LB6 5LBB
• 分子名称: Egl nine homolog 1, MANGANESE (II) ION, CHLORIDE ION, ... (8 entities in total)
• 機能のキーワード: oxidoreductase, non-heme dioxygenase, iron, 2-oxoglutarate, hypoxia-inducible factor, hif, hif prolyl hydroxylase domain 2, phd2, egln1, oxygenase, hypoxia, dna-binding, metal-binding, transcription, helix-loop-helix-beta, dsbh, facial triad, cytoplasm, transcription/epigenetic regulation, signaling, development, cell structure, beta-hydroxylation, transcription activator/inhibitor, ubl conjugation, polymorphism, vitamin c, zinc-finger, familial erythrocytosis, breast cancer, transcription complex
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Cytoplasm : Q9GZT9
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28833.24

構造登録者

Chowdhury, R.,Schofield, C.J. (登録日: 2016-06-15, 公開日: 2016-08-31, 最終更新日: 2024-01-10)

主引用文献

Chowdhury, R.,Leung, I.K.,Tian, Y.M.,Abboud, M.I.,Ge, W.,Domene, C.,Cantrelle, F.X.,Landrieu, I.,Hardy, A.P.,Pugh, C.W.,Ratcliffe, P.J.,Claridge, T.D.,Schofield, C.J.
Structural basis for oxygen degradation domain selectivity of the HIF prolyl hydroxylases.
Nat Commun, 7:12673-12673, 2016

PubMed Abstract: The response to hypoxia in animals involves the expression of multiple genes regulated by the αβ-hypoxia-inducible transcription factors (HIFs). The hypoxia-sensing mechanism involves oxygen limited hydroxylation of prolyl residues in the N- and C-terminal oxygen-dependent degradation domains (NODD and CODD) of HIFα isoforms, as catalysed by prolyl hydroxylases (PHD 1-3). Prolyl hydroxylation promotes binding of HIFα to the von Hippel-Lindau protein (VHL)-elongin B/C complex, thus signalling for proteosomal degradation of HIFα. We reveal that certain PHD2 variants linked to familial erythrocytosis and cancer are highly selective for CODD or NODD. Crystalline and solution state studies coupled to kinetic and cellular analyses reveal how wild-type and variant PHDs achieve ODD selectivity via different dynamic interactions involving loop and C-terminal regions. The results inform on how HIF target gene selectivity is achieved and will be of use in developing selective PHD inhibitors.

PubMed: 27561929
DOI: 10.1038/ncomms12673

実験手法

X-RAY DIFFRACTION (1.816 Å)