5LBC
HIF prolyl hydroxylase 2 (PHD2/EGLN1) I280V/R281L/I292V variant in complex with Mn(II) and N-[(1-chloro-4-hydroxyisoquinolin-3-yl)carbonyl]glycine (IOX3/FG2216)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Egl nine homolog 1 | polymer | 252 | 28024.9 | 1 | UniProt (Q9GZT9) Pfam (PF13640) | Homo sapiens (Human) | Hypoxia-inducible factor prolyl hydroxylase 2,HPH-2,Prolyl hydroxylase domain-containing protein 2,PHD2,SM-20 |
| 2 | B (A) | MANGANESE (II) ION | non-polymer | 54.9 | 1 | Chemie (MN) | |||
| 3 | C (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 4 | D (A) | N-[(1-CHLORO-4-HYDROXYISOQUINOLIN-3-YL)CARBONYL]GLYCINE | non-polymer | 280.7 | 1 | Chemie (UN9) | |||
| 5 | E, H (A) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 6 | F, G (A) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 7 | I (A) | BICARBONATE ION | non-polymer | 61.0 | 1 | Chemie (BCT) | |||
| 8 | J (A) | water | water | 18.0 | 138 | Chemie (HOH) |
Sequence modifications
A: 181 - 426 (UniProt: Q9GZT9)
| PDB | External Database | Details |
|---|---|---|
| Gly 175 | - | expression tag |
| Ser 176 | - | expression tag |
| His 177 | - | expression tag |
| Met 178 | - | expression tag |
| Ala 179 | - | expression tag |
| Ser 180 | - | expression tag |
| Val 280 | Ile 280 | engineered mutation |
| Leu 281 | Arg 281 | engineered mutation |
| Val 292 | Ile 292 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 28024.9 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 808.4 | |
| All* | Total formula weight | 28833.2 |
