5KSC

E166A/R274N/R276N Toho-1 Beta-lactamase aztreonam acyl-enzyme intermediate

Summary for 5KSC

• Entry DOI: 10.2210/pdb5ksc/pdb
• Descriptor: Beta-lactamase Toho-1, 2-({[(1Z)-1-(2-amino-1,3-thiazol-4-yl)-2-oxo-2-{[(2S,3S)-1-oxo-3-(sulfoamino)butan-2-yl]amino}ethylidene]amino}oxy)-2-methylpropanoic acid (3 entities in total)
• Functional Keywords: beta-lactamase, aztreonam, acyl-intermediate, hydrolase
• Biological source: Escherichia coli
• Total number of polymer chains: 1
• Total formula weight: 28484.13

Authors

Vandavasi, V.G.,Langan, P.S.,Weiss, K.,Parks, J.M.,Cooper, J.B.,Ginell, S.L.,Coates, L. (deposition date: 2016-07-08, release date: 2016-11-09, Last modification date: 2019-12-04)

• Primary citation: Vandavasi, V.G.,Langan, P.S.,Weiss, K.L.,Parks, J.M.,Cooper, J.B.,Ginell, S.L.,Coates, L.; Active-Site Protonation States in an Acyl-Enzyme Intermediate of a Class A beta-Lactamase with a Monobactam Substrate.; Antimicrob. Agents Chemother., 61:-, 2017; PubMed: 27795378; DOI: 10.1128/AAC.01636-16

Experimental method

NEUTRON DIFFRACTION (2.1 Å)