5KG4

Human DNA polymerase eta-DNA ternary complex: reaction first with 1 mM Mn2+ for 1800s then with 10 mM Mg2+ for 60s

Summary for 5KG4

Related5KFA 5KFB 5KFC 5KG0 5KG1 5KG2 5KG3 5KG5 5KG6 5KG7 5KFD 5KFE 5KFS 5KFT 5KFU 5KFV 5KFW 5KFX 5KFG 5KFY 5KFZ 5KFH 5KFK 5KFL 5KFI 5KFJ 5KFM 5KFN 5KFO 5KFP 5KFQ 5KFR 5KFF
DescriptorDNA polymerase eta, DNA (5'-D(*CP*AP*TP*TP*AP*TP*GP*AP*CP*GP*CP*T)-3'), DNA (5'-D(*AP*GP*CP*GP*TP*CP*AP*TP*A)-3'), ... (9 entities in total)
Functional Keywordsin crystallo reaction, dna polymerase, metal ion dependent catalysis, replication, transferase-dna complex, transferase/dna
Biological sourceHomo sapiens (Human)
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Cellular locationNucleus  Q9Y253
Total number of polymer chains3
Total molecular weight56070.51
Authors
Gao, Y.,Yang, W. (deposition date: 2016-06-12, release date: 2016-06-22, Last modification date: 2017-09-20)
Primary citation
Gao, Y.,Yang, W.
Capture of a third Mg2+ is essential for catalyzing DNA synthesis.
Science, 352:1334-1337, 2016
PubMed: 27284197 (PDB entries with the same primary citation)
DOI: 10.1126/science.aad9633
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.6 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.223501.6%7.5%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution