5KEG

Crystal structure of APOBEC3A in complex with a single-stranded DNA

5KEG の概要

• エントリーDOI: 10.2210/pdb5keg/pdb
• 分子名称: DNA dC->dU-editing enzyme APOBEC-3A, DNA (5'-D(*TP*TP*CP*TP*T)-3'), ZINC ION, ... (6 entities in total)
• 機能のキーワード: apobec3a, dna cytidine deaminase complex with a substrate dna, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27944.14

構造登録者

Kouno, T.,Hilbert, B.J.,Silvas, T.,Royer, W.E.,Matsuo, H.,Schiffer, C.A. (登録日: 2016-06-09, 公開日: 2017-05-10, 最終更新日: 2024-10-16)

主引用文献

Kouno, T.,Silvas, T.V.,Hilbert, B.J.,Shandilya, S.M.D.,Bohn, M.F.,Kelch, B.A.,Royer, W.E.,Somasundaran, M.,Kurt Yilmaz, N.,Matsuo, H.,Schiffer, C.A.
Crystal structure of APOBEC3A bound to single-stranded DNA reveals structural basis for cytidine deamination and specificity.
Nat Commun, 8:15024-15024, 2017

PubMed Abstract: Nucleic acid editing enzymes are essential components of the immune system that lethally mutate viral pathogens and somatically mutate immunoglobulins, and contribute to the diversification and lethality of cancers. Among these enzymes are the seven human APOBEC3 deoxycytidine deaminases, each with unique target sequence specificity and subcellular localization. While the enzymology and biological consequences have been extensively studied, the mechanism by which APOBEC3s recognize and edit DNA remains elusive. Here we present the crystal structure of a complex of a cytidine deaminase with ssDNA bound in the active site at 2.2 Å. This structure not only visualizes the active site poised for catalysis of APOBEC3A, but pinpoints the residues that confer specificity towards CC/TC motifs. The APOBEC3A-ssDNA complex defines the 5'-3' directionality and subtle conformational changes that clench the ssDNA within the binding groove, revealing the architecture and mechanism of ssDNA recognition that is likely conserved among all polynucleotide deaminases, thereby opening the door for the design of mechanistic-based therapeutics.

PubMed: 28452355
DOI: 10.1038/ncomms15024

実験手法

X-RAY DIFFRACTION (2.2 Å)