5KDL

Crystal structure of the 4 alanine insertion variant of the Gi alpha1 subunit bound to GTPgammaS

Summary for 5KDL

Related5KDO
DescriptorGuanine nucleotide-binding protein G(i) subunit alpha-1, MAGNESIUM ION, 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE, ... (4 entities in total)
Functional Keywordsheterotrimeric g protein g protein coupled receptors g protein activation g protein structure gdp release, signaling protein
Biological sourceRattus norvegicus (Rat)
Cellular locationNucleus P10824
Total number of polymer chains2
Total molecular weight82493.8
Authors
Kaya, A.I.,Lokits, A.D.,Gilbert, J.,Iverson, T.M.,Meiler, J.,Hamm, H.E. (deposition date: 2016-06-08, release date: 2016-08-03, Last modification date: 2017-09-13)
Primary citation
Kaya, A.I.,Lokits, A.D.,Gilbert, J.A.,Iverson, T.M.,Meiler, J.,Hamm, H.E.
A Conserved Hydrophobic Core in G alpha i1 Regulates G Protein Activation and Release from Activated Receptor.
J.Biol.Chem., 291:19674-19686, 2016
PubMed: 27462082 (PDB entries with the same primary citation)
DOI: 10.1074/jbc.M116.745513
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.665 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.2617003.4%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

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More Biological unit images

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Molmil generated image of 5kdl
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(*)In the case of coarse surface representation, the asymmetric unit is shown as red ribbon representation.
Coordinate files for Biological unit (5kdl.pdb1.gz [53.01 KB])
Coordinate files for Biological unit (5kdl.pdb2.gz [53.77 KB])