5KDL
Crystal structure of the 4 alanine insertion variant of the Gi alpha1 subunit bound to GTPgammaS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.742, 77.351, 73.095 |
| Unit cell angles | 90.00, 99.86, 90.00 |
Refinement procedure
| Resolution | 47.816 - 2.665 |
| R-factor | 0.2148 |
| Rwork | 0.209 |
| R-free | 0.26470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gia |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.541 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.816 | 2.800 |
| High resolution limit [Å] | 2.660 | 2.660 |
| Number of reflections | 19135 | |
| <I/σ(I)> | 14.4 | 2.04 |
| Completeness [%] | 99.5 | 97.4 |
| Redundancy | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 12-16 % polyethylene glycol (PEG) 2000 monomethyl ether, 18% 2-propanol and 100 mM MES (pH 6.0) |
