5JUY
Active human apoptosome with procaspase-9
Summary for 5JUY
| Entry DOI | 10.2210/pdb5juy/pdb |
| EMDB information | 8178 |
| Descriptor | Apoptotic protease-activating factor 1, Cytochrome c, Caspase-9, ... (5 entities in total) |
| Functional Keywords | apaf-1, apoptosis, programmed cell death, aaa+ atpase |
| Biological source | Homo sapiens (Human) More |
| Total number of polymer chains | 18 |
| Total formula weight | 1127664.13 |
| Authors | Cheng, T.C.,Hong, C.,Akey, I.V.,Yuan, S.,Akey, C.W. (deposition date: 2016-05-10, release date: 2016-10-19, Last modification date: 2024-11-06) |
| Primary citation | Cheng, T.C.,Hong, C.,Akey, I.V.,Yuan, S.,Akey, C.W. A near atomic structure of the active human apoptosome. Elife, 5:-, 2016 Cited by PubMed Abstract: In response to cell death signals, an active apoptosome is assembled from Apaf-1 and procaspase-9 (pc-9). Here we report a near atomic structure of the active human apoptosome determined by cryo-electron microscopy. The resulting model gives insights into cytochrome c binding, nucleotide exchange and conformational changes that drive assembly. During activation an acentric disk is formed on the central hub of the apoptosome. This disk contains four Apaf-1/pc-9 CARD pairs arranged in a shallow spiral with the fourth pc-9 CARD at lower occupancy. On average, Apaf-1 CARDs recruit 3 to 5 pc-9 molecules to the apoptosome and one catalytic domain may be parked on the hub, when an odd number of zymogens are bound. This suggests a stoichiometry of one or at most, two pc-9 dimers per active apoptosome. Thus, our structure provides a molecular framework to understand the role of the apoptosome in programmed cell death and disease. PubMed: 27697150DOI: 10.7554/eLife.17755 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (4.1 Å) |
Structure validation
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