5JUT

Saccharomyces cerevisiae 80S ribosome bound with elongation factor eEF2-GDP-sordarin and Taura Syndrome Virus IRES, Structure IV (almost non-rotated 40S subunit)

これはPDB形式変換不可エントリーです。

5JUT の概要

• エントリーDOI: 10.2210/pdb5jut/pdb
• 関連するPDBエントリー: 5JUO 5JUP 5JUS 5JUU
• EMDBエントリー: 6643 6644 6645 6646 6647 6648 6649 6650 6652 6653
• 分子名称: 18S ribosomal RNA, eL6 (yeast L6), uL30 (yeast L7), ... (86 entities in total)
• 機能のキーワード: 80s-ires, eef2, translocation, sordarin, ribosome
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 83
• 化学式量合計: 3340490.76

構造登録者

Abeyrathne, P.,Koh, C.S.,Grant, T.,Grigorieff, N.,Korostelev, A.A. (登録日: 2016-05-10, 公開日: 2016-10-05, 最終更新日: 2025-04-02)

主引用文献

Abeyrathne, P.D.,Koh, C.S.,Grant, T.,Grigorieff, N.,Korostelev, A.A.
Ensemble cryo-EM uncovers inchworm-like translocation of a viral IRES through the ribosome.
Elife, 5:-, 2016

PubMed Abstract: Internal ribosome entry sites (IRESs) mediate cap-independent translation of viral mRNAs. Using electron cryo-microscopy of a single specimen, we present five ribosome structures formed with the Taura syndrome virus IRES and translocase eEF2•GTP bound with sordarin. The structures suggest a trajectory of IRES translocation, required for translation initiation, and provide an unprecedented view of eEF2 dynamics. The IRES rearranges from extended to bent to extended conformations. This inchworm-like movement is coupled with ribosomal inter-subunit rotation and 40S head swivel. eEF2, attached to the 60S subunit, slides along the rotating 40S subunit to enter the A site. Its diphthamide-bearing tip at domain IV separates the tRNA-mRNA-like pseudoknot I (PKI) of the IRES from the decoding center. This unlocks 40S domains, facilitating head swivel and biasing IRES translocation via hitherto-elusive intermediates with PKI captured between the A and P sites. The structures suggest missing links in our understanding of tRNA translocation.

PubMed: 27159452
DOI: 10.7554/eLife.14874

実験手法

ELECTRON MICROSCOPY (4 Å)