5J6X

Crystal structure of the apo-Zalpha of Zebrafish PKZ

Summary for 5J6X

Entry DOI	10.2210/pdb5j6x/pdb
Descriptor	Z-DNA binding protein kinase (2 entities in total)
Functional Keywords	pkz zdna zalpha whth, dna binding protein
Biological source	Danio rerio (Zebrafish)
Total number of polymer chains	2
Total formula weight	17075.58
Authors	Subramani, V.K.,Kim, D.,Kim, K.K. (deposition date: 2016-04-05, release date: 2016-09-07, Last modification date: 2023-11-08)
Primary citation	Subramani, V.K.,Kim, D.,Yun, K.,Kim, K.K. Structural and functional studies of a large winged Z-DNA-binding domain of Danio rerio protein kinase PKZ Febs Lett., 590:2275-2285, 2016 Cited by PubMed Abstract: The Z-DNA-binding domain of PKZ from zebrafish (Danio rerio; drZαPKZ ) contains the largest β-wing among known Z-DNA-binding domains. To elucidate the functional implication of the β-wing, we solved the crystal structure of apo-drZαPKZ . Structural comparison with its Z-DNA-bound form revealed a large conformational change within the β-wing during Z-DNA binding. Biochemical studies of protein mutants revealed that two basic residues in the β-wing are responsible for Z-DNA recognition as well as fast B-Z transition. Therefore, the extra basic residues in the β-wing of drZαPKZ are necessary for the fast B-Z transition activity. PubMed: 27265117 DOI: 10.1002/1873-3468.12238 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.493 Å)

Structure validation