5J6X
Crystal structure of the apo-Zalpha of Zebrafish PKZ
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-27 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.000 |
Spacegroup name | P 41 |
Unit cell lengths | 44.304, 44.304, 71.369 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.686 - 2.493 |
R-factor | 0.1915 |
Rwork | 0.182 |
R-free | 0.26890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4kmf |
RMSD bond length | 0.009 |
RMSD bond angle | 1.107 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 28.690 |
High resolution limit [Å] | 2.490 |
Rmerge | 0.152 |
Number of reflections | 4804 |
<I/σ(I)> | 35.98 |
Completeness [%] | 98.9 |
Redundancy | 11.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.5 | 277.15 | High concentration (3mM) of the purified protein in 20 mM HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid], 80 mM Sodium chloride was stored at 4degC. Crystals were cryo-protected with 35% (v/v) PEG400. |