5IX6

X-ray structure of Auoxo3-encapsulated horse spleen apoferritin

Summary for 5IX6

• Entry DOI: 10.2210/pdb5ix6/pdb
• Related: 5ERK
• Descriptor: Ferritin light chain, GOLD ION, CADMIUM ION, ... (5 entities in total)
• Functional Keywords: ferritin, nanocage, metallodrugs, gold compounds, transport protein
• Biological source: Equus caballus (Horse)
• Total number of polymer chains: 1
• Total formula weight: 22358.96

Authors

Ferraro, G.,Pontillo, N.,Merlino, A. (deposition date: 2016-03-23, release date: 2016-07-06, Last modification date: 2024-01-10)

Primary citation

Ferraro, G.,Monti, D.M.,Amoresano, A.,Pontillo, N.,Petruk, G.,Pane, F.,Cinellu, M.A.,Merlino, A.
Gold-based drug encapsulation within a ferritin nanocage: X-ray structure and biological evaluation as a potential anticancer agent of the Auoxo3-loaded protein.
Chem.Commun.(Camb.), 52:9518-9521, 2016

PubMed Abstract: Auoxo3, a cytotoxic gold(iii) compound, was encapsulated within a ferritin nanocage. Inductively coupled plasma mass spectrometry, circular dichroism, UV-Vis absorption spectroscopy and X-ray crystallography confirm the potential-drug encapsulation. The structure shows that naked Au(i) ions bind to the side chains of Cys48, His49, His114, His114 and Cys126, Cys126, His132, His147. The gold-encapsulated nanocarrier has a cytotoxic effect on different aggressive human cancer cells, whereas it is significantly less cytotoxic for non-tumorigenic cells.

PubMed: 27326513
DOI: 10.1039/c6cc02516a

Experimental method

X-RAY DIFFRACTION (1.85 Å)