SigmaS-transcription initiation complex with 4-nt nascent RNA

Summary for 5IPN

Related5IPL 5IPM
DescriptorDNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (10 entities in total)
Functional Keywordstranscription initiation, rna polymerase, general stress sigma factor, pyrophosphate release, transcription, transferase-dna-rna complex, transferase/dna/rna
Biological sourceEscherichia coli
Cellular locationCytoplasm  P13445
Total number of polymer chains9
Total molecular weight441356.43
Liu, B.,Zuo, Y.,Steitz, T.A. (deposition date: 2016-03-09, release date: 2016-03-30, Last modification date: 2016-06-22)
Primary citation
Liu, B.,Zuo, Y.,Steitz, T.A.
Structures of E. coli sigma S-transcription initiation complexes provide new insights into polymerase mechanism.
Proc.Natl.Acad.Sci.USA, 113:4051-4056, 2016
PubMed: 27035955 (PDB entries with the same primary citation)
DOI: 10.1073/pnas.1520555113
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone 0.32715 1.1% 2.0% 3.8% 0.00MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
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PDB entries from 2020-11-18