5IPN
SigmaS-transcription initiation complex with 4-nt nascent RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97919 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 132.874, 152.170, 229.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 126.760 - 4.610 |
R-factor | 0.28316 |
Rwork | 0.280 |
R-free | 0.34466 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.371 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 126.760 | |
High resolution limit [Å] | 4.600 | 4.600 |
Number of reflections | 24772 | |
<I/σ(I)> | 10.06 | |
Completeness [%] | 99.3 | |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 295 | PEG3350, sodium chloride, HEPES |