5IM4

Crystal structure of designed two-component self-assembling icosahedral cage I52-32

Summary for 5IM4

Descriptor6,7-dimethyl-8-ribityllumazine synthase, Phosphotransferase system, mannose/fructose-specific component IIA (2 entities in total)
Functional Keywordsicosahedron, designed protein cage, two-component, computational design, protein engineering, rosetta, self-assembling, co-assembling, multimerization, symmetry, nanomaterial, nanostructure, protein binding
Biological sourceCandida albicans P37005
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Total number of polymer chains40
Total molecular weight665301.76
Authors
Liu, Y.A.,Cascio, D.,Sawaya, M.R.,Bale, J.B.,Collazo, M.J.,Thomas, C.,Sheffler, W.,King, N.P.,Baker, D.,Yeates, T.O. (deposition date: 2016-03-05, release date: 2016-07-27, Last modification date: 2017-11-01)
Primary citation
Bale, J.B.,Gonen, S.,Liu, Y.,Sheffler, W.,Ellis, D.,Thomas, C.,Cascio, D.,Yeates, T.O.,Gonen, T.,King, N.P.,Baker, D.
Accurate design of megadalton-scale two-component icosahedral protein complexes.
Science, 353:389-394, 2016
PubMed: 27463675 (PDB entries with the same primary citation)
DOI: 10.1126/science.aaf8818
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3.5 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.234903.5%3.3%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution