5IFA

Crystal structure of unbound VRC01c-HuGL2 Fab from an HIV-1 naive donor at 1.82 A

5IFA の概要

• エントリーDOI: 10.2210/pdb5ifa/pdb
• 関連するPDBエントリー: 5IES 5IF0
• 分子名称: VRC01c-HuGL2 Fab heavy chain, VRC01c-HuGL2 Fab light chain, SULFATE ION, ... (6 entities in total)
• 機能のキーワード: vrc01-class naive human germline antibody, cd4 binding site, immune system, anti-hiv-1
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49316.88

構造登録者

Sarkar, A.,Wilson, I.A. (登録日: 2016-02-25, 公開日: 2016-04-06, 最終更新日: 2024-12-25)

主引用文献

Jardine, J.G.,Kulp, D.W.,Havenar-Daughton, C.,Sarkar, A.,Briney, B.,Sok, D.,Sesterhenn, F.,Ereno-Orbea, J.,Kalyuzhniy, O.,Deresa, I.,Hu, X.,Spencer, S.,Jones, M.,Georgeson, E.,Adachi, Y.,Kubitz, M.,deCamp, A.C.,Julien, J.P.,Wilson, I.A.,Burton, D.R.,Crotty, S.,Schief, W.R.
HIV-1 broadly neutralizing antibody precursor B cells revealed by germline-targeting immunogen.
Science, 351:1458-1463, 2016

PubMed Abstract: Induction of broadly neutralizing antibodies (bnAbs) is a major HIV vaccine goal. Germline-targeting immunogens aim to initiate bnAb induction by activating bnAb germline precursor B cells. Critical unmet challenges are to determine whether bnAb precursor naïve B cells bind germline-targeting immunogens and occur at sufficient frequency in humans for reliable vaccine responses. Using deep mutational scanning and multitarget optimization, we developed a germline-targeting immunogen (eOD-GT8) for diverse VRC01-class bnAbs. We then used the immunogen to isolate VRC01-class precursor naïve B cells from HIV-uninfected donors. Frequencies of true VRC01-class precursors, their structures, and their eOD-GT8 affinities support this immunogen as a candidate human vaccine prime. These methods could be applied to germline targeting for other classes of HIV bnAbs and for Abs to other pathogens.

PubMed: 27013733
DOI: 10.1126/science.aad9195

実験手法

X-RAY DIFFRACTION (1.821 Å)