5I4T

Immature hexagonal lattice of HIV-1 Gag

Summary for 5I4T

• Entry DOI: 10.2210/pdb5i4t/pdb
• Descriptor: CTD-SP1 fragment of HIV-1 Gag (1 entity in total)
• Functional Keywords: capsid, immature hexagonal lattice, viral protein
• Biological source: Human immunodeficiency virus 1
• Total number of polymer chains: 6
• Total formula weight: 73157.37

Authors

Pornillos, O.,Wagner, J.M.,Zadrozny, K.,Ganser-Pornillos, B.K. (deposition date: 2016-02-12, release date: 2016-07-27, Last modification date: 2023-09-27)

Primary citation

Wagner, J.M.,Zadrozny, K.K.,Chrustowicz, J.,Purdy, M.D.,Yeager, M.,Ganser-Pornillos, B.K.,Pornillos, O.
Crystal structure of an HIV assembly and maturation switch.
Elife, 5:-, 2016

PubMed Abstract: Virus assembly and maturation proceed through the programmed operation of molecular switches, which trigger both local and global structural rearrangements to produce infectious particles. HIV-1 contains an assembly and maturation switch that spans the C-terminal domain (CTD) of the capsid (CA) region and the first spacer peptide (SP1) of the precursor structural protein, Gag. The crystal structure of the CTD-SP1 Gag fragment is a goblet-shaped hexamer in which the cup comprises the CTD and an ensuing type II β-turn, and the stem comprises a 6-helix bundle. The β-turn is critical for immature virus assembly and the 6-helix bundle regulates proteolysis during maturation. This bipartite character explains why the SP1 spacer is a critical element of HIV-1 Gag but is not a universal property of retroviruses. Our results also indicate that HIV-1 maturation inhibitors suppress unfolding of the CA-SP1 junction and thereby delay access of the viral protease to its substrate.

PubMed: 27416583
DOI: 10.7554/eLife.17063

Experimental method

X-RAY DIFFRACTION (3.273 Å)