5I4T
Immature hexagonal lattice of HIV-1 Gag
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-15 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.959, 122.733, 85.414 |
| Unit cell angles | 90.00, 94.30, 90.00 |
Refinement procedure
| Resolution | 42.587 - 3.273 |
| R-factor | 0.2498 |
| Rwork | 0.246 |
| R-free | 0.27830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4usn |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.513 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.273 | 3.273 |
| Rmerge | 0.740 | |
| Number of reflections | 9732 | |
| <I/σ(I)> | 5.986 | 1.3 |
| Completeness [%] | 87.0 | 66.4 |
| Redundancy | 3.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.8 M LiSO4 0.1 M Bis-Tris propane |
