5HQ3

Stable, high-expression variant of human acetylcholinesterase

Summary for 5HQ3

• Entry DOI: 10.2210/pdb5hq3/pdb
• Descriptor: Acetylcholinesterase, O-ETHYLMETHYLPHOSPHONIC ACID ESTER GROUP, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, ... (5 entities in total)
• Functional Keywords: design, de novo protein, hydrolase
• Biological source: Homo sapiens (Human); More
• Cellular location: Cell junction, synapse . Isoform T: Nucleus. Isoform H: Cell membrane ; Lipid-anchor, GPI-anchor ; Extracellular side : P22303 P22303
• Total number of polymer chains: 2
• Total formula weight: 123155.34

Authors

Goldenzweig, A.,Goldsmith, M.,Hill, S.E.,Gertman, O.,Laurino, P.,Ashani, Y.,Dym, O.,Albeck, S.,Unger, T.,Prilusky, J.,Lieberman, R.L.,Aharoni, A.,Silman, I.,Sussman, J.L.,Tawfik, D.S.,Fleishman, S.J. (deposition date: 2016-01-21, release date: 2016-07-27, Last modification date: 2024-01-10)

• Primary citation: Goldenzweig, A.,Goldsmith, M.,Hill, S.E.,Gertman, O.,Laurino, P.,Ashani, Y.,Dym, O.,Unger, T.,Albeck, S.,Prilusky, J.,Lieberman, R.L.,Aharoni, A.,Silman, I.,Sussman, J.L.,Tawfik, D.S.,Fleishman, S.J.; Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability.; Mol.Cell, 63:337-346, 2016; PubMed: 27425410; DOI: 10.1016/j.molcel.2016.06.012

Experimental method

X-RAY DIFFRACTION (2.6 Å)