5HHK

Reverse transcriptase domain of group II intron maturase from Roseburia intestinalis in P21 space group (Se-MET)

Summary for 5HHK

• Entry DOI: 10.2210/pdb5hhk/pdb
• Related: 5HHJ 5HHL
• Descriptor: Retron-type reverse transcriptase, PHOSPHATE ION, POTASSIUM ION, ... (6 entities in total)
• Functional Keywords: group ii intron, rna binding protein
• Biological source: Roseburia intestinalis XB6B4
• Total number of polymer chains: 2
• Total formula weight: 72025.80

Authors

Zhao, C.,Pyle, A.M. (deposition date: 2016-01-11, release date: 2016-05-04, Last modification date: 2024-11-13)

Primary citation

Zhao, C.,Pyle, A.M.
Crystal structures of a group II intron maturase reveal a missing link in spliceosome evolution.
Nat.Struct.Mol.Biol., 23:558-565, 2016

PubMed Abstract: Group II introns are self-splicing ribozymes that are essential in many organisms, and they have been hypothesized to share a common evolutionary ancestor with the spliceosome. Although structural similarity of RNA components supports this connection, it is of interest to determine whether associated protein factors also share an evolutionary heritage. Here we present the crystal structures of reverse transcriptase (RT) domains from two group II intron-encoded proteins (maturases) from Roseburia intestinalis and Eubacterium rectale, obtained at 1.2-Å and 2.1-Å resolution, respectively. These domains are more similar in architecture to the spliceosomal Prp8 RT-like domain than to any other RTs, and they share substantial similarity with flaviviral RNA polymerases. The RT domain itself is sufficient for binding intron RNA with high affinity and specificity, and it is contained within an active RT enzyme. These studies provide a foundation for understanding structure-function relationships within group II intron-maturase complexes.

PubMed: 27136328
DOI: 10.1038/nsmb.3224

Experimental method

X-RAY DIFFRACTION (1.4 Å)