5HHK
Reverse transcriptase domain of group II intron maturase from Roseburia intestinalis in P21 space group (Se-MET)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.010, 88.120, 79.670 |
Unit cell angles | 90.00, 95.45, 90.00 |
Refinement procedure
Resolution | 44.060 - 1.400 |
R-factor | 0.159 |
Rwork | 0.157 |
R-free | 0.18940 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 0.984 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXCD |
Refinement software | PHENIX ((dev_2254: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 44.060 |
High resolution limit [Å] | 1.400 |
Number of reflections | 104342 |
<I/σ(I)> | 13.5 |
Completeness [%] | 98.7 |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291.15 | 100 mM SPG pH 9.0, 23 % PEG 1500 |