5HHK
Reverse transcriptase domain of group II intron maturase from Roseburia intestinalis in P21 space group (Se-MET)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.010, 88.120, 79.670 |
| Unit cell angles | 90.00, 95.45, 90.00 |
Refinement procedure
| Resolution | 44.060 - 1.400 |
| R-factor | 0.159 |
| Rwork | 0.157 |
| R-free | 0.18940 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.984 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXCD |
| Refinement software | PHENIX ((dev_2254: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.060 |
| High resolution limit [Å] | 1.400 |
| Number of reflections | 104342 |
| <I/σ(I)> | 13.5 |
| Completeness [%] | 98.7 |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291.15 | 100 mM SPG pH 9.0, 23 % PEG 1500 |
