5HH3

OxyA from Actinoplanes teichomyceticus

Summary for 5HH3

• Entry DOI: 10.2210/pdb5hh3/pdb
• Descriptor: OxyA protein, PROTOPORPHYRIN IX CONTAINING FE, GLYCEROL, ... (5 entities in total)
• Functional Keywords: cytochrome p450 monooxygenase, phenolic coupling enzyme, biosynthesis of glycopeptide antibiotics, peptide cyclase, oxidoreductase
• Biological source: Actinoplanes teichomyceticus
• Total number of polymer chains: 2
• Total formula weight: 91189.51

Authors

Haslinger, K.,Cryle, M.J. (deposition date: 2016-01-09, release date: 2016-02-10, Last modification date: 2024-01-10)

Primary citation

Haslinger, K.,Cryle, M.J.
Structure of OxyAtei : completing our picture of the glycopeptide antibiotic producing Cytochrome P450 cascade.
Febs Lett., 590:571-581, 2016

PubMed Abstract: Cyclization of glycopeptide antibiotic precursors occurs in either three or four steps catalyzed by Cytochrome P450 enzymes. Three of these enzymes have been structurally characterized to date with the second enzyme along the pathway, OxyA, escaping structural analysis. We are now able to present the structure of OxyAtei involved in teicoplanin biosynthesis - the same enzyme recently shown to be the first active OxyA homolog. In spite of the hydrophobic character of the teicoplanin precursor, the polar active site of OxyAtei and its affinity for certain azole inhibitors hint at its preference for substrates with polar decorations.

PubMed: 26820384
DOI: 10.1002/1873-3468.12081

Experimental method

X-RAY DIFFRACTION (2.1 Å)